MCF10A-Z Cell Line in Co-Culture as In vitro Model of Mammary Epithelial Drug Transport
Many drugs ingested by lactating women are transported into breastmilk and ingested by the infant. To predict the amount in milk, research focuses on developing in vitro models of mammary epithelial drug transport. The mammary epithelium is composed of distinct cell layers; inner, secretory luminal cells and outer, contractile basal cells. This study aims to co-culture two cell lines, a luminal and basal/myoepithelial line, to model drug permeability in the mammary epithelium. Use of the novel line, MCF10A-Z, as a luminal cell in co-culture with basal/myoepithelial cells will be assessed. Unlike the original MCF10A (a mammary line widely used for breast cell study), MCF10A-Z expresses tight junctions with zonula occluden (ZO-1) staining. TJs play a significant transport role, preventing paracellular transport, regulating epithelial transport, and suggesting luminal character. MCF10A-Z TJ function may be more representative of in vivo, making it an ideal luminal line for co-culture to predict drug permeability.