Publication Date
2025
Document Type
Book
Description
Brook trout (Salvelinus fontinalis), a predatory freshwater species, often consume prey fish with elevated levels of thiaminase. Previous studies have shown that thiaminase degrades thiamine (Vitamin B1), an essential cofactor in metabolic pathways including the pentose-phosphate pathway, citric acid cycle, and glycolysis. The deficiency of thiamine can impair RNA and DNA synthesis due to reduced NADPH production. This study investigated the impact of environmental factors on the thiamine levels in the diets of these brook trouts. Six brook trout strains from New York State and Canada, living in temperatures of 4°C and 19°C, and some under hypoxic conditions, were examined. A transketolase activity assay was developed and applied to fish liver samples for thiamine analysis. The findings are significant, as the assay paves the way for further studies on cellular redox states, oxidative stress, and the potential adverse effects of regulated enzymes on fish health and development.
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Recommended Citation
Mangal, Banshika and Kleiner, Dennis, "Effects of Environmental Conditions on Thiamine Levels and Health of Brook Trout" (2025). Research Days Posters 2025. 111.
https://orb.binghamton.edu/research_days_posters_2025/111
