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This study evaluated behaviors and monoamine levels of the neonatal clomipramine (neoCLOM) model of Obsessive Compulsive Disorder (OCD) in male and female rats (36 of each). Subjects were injected with 15 mg/kg of the serotonin-norepinephrine uptake inhibitor clomipramine during a developmentally sensitive period. A unique combination of Hole Board (HB) and Elevated Plus Maze (EPM) apparatuses was used to evaluate compulsiveness and anxiety. There was a significant effect of Treatment in the HB. Male neoCLOMs had increased hole poke and repeats versus control male neoSALs. In contrast, there was a significant effect of Sex in the EPM. Female neoCLOMs spent more time in open arms than male neoCLOMs. HB and EPM behaviors did not correlate for any group. Serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels in post mortem tissue homogenates from the hypothalamus and amygdala were analyzed using High Performance Liquid Chromatography. There were significant effects of Treatment and Sex. Neurochemical abnormalities reflect monoamine dysfunction in OCD patients. Results support some aspects of the face and construct validity of the model. Further research is needed to evaluate the model's predictive validity, sensitivity to sex differences, and potential usefulness in identification of new treatment methods for OCD patients.



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Neonatal Clomipramine Model of Obsessive-Compulsive Disorder (OCD) Demonstrates Treatment and Sex Differences