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The objective of this study was to evaluate the reliability of data from the assay of bio-archived (frozen long-term) specimens. A 50-freeze-thaw-cycle degradation study of fresh sera was conducted to test the stability of 16 immunoregulators. These include C-reactive protein (CRP), interleukin (IL)-1a, 4, 6, 8, 10, monocyte chemoattractant protein-1 (MCP-1, CCL2), monocyte chemoattractant protein-2 (MCP-2, CCL8), eotaxin-1, thymus-and-activation-regulated chemokine (TARC, CCL17), regulated on activation normal T cell expressed and secreted (RANTES, CCL5), growth-regulated alpha (GRO-α, CXCL1), small inducible cytokine A1 (I-309, CCL1), interferon-gamma (IFN-γ), interferon-gamma inducible protein-10 (IP-10, CXCL10), and tumor necrosis factor (TNF-a).
Twenty de-identified serum specimens were obtained from volunteers at UHS-Wilson Memorial Hospital. Samples were stored at -20°C and underwent daily 1-hour thawing and subsequent freezing for each freeze-thaw cycle (FTC) over 49 consecutive days. Immunoregulator concentrations were assessed in participant samples at 2FTC (baseline), 25FTC, and 50FTC.
Chowdhury, Risana N., "Immunoregulator stability/Freeze-thaw-cycle validation study data set 1" (2022). Anthropology Datasets. 10.
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