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The objective of this study was to evaluate the reliability of data from the assay of bio-archived (frozen long-term) specimens. A 50-freeze-thaw-cycle degradation study of fresh sera was conducted to test the stability of 16 immunoregulators. These include C-reactive protein (CRP), interleukin (IL)-1a, 4, 6, 8, 10, monocyte chemoattractant protein-1 (MCP-1, CCL2), monocyte chemoattractant protein-2 (MCP-2, CCL8), eotaxin-1, thymus-and-activation-regulated chemokine (TARC, CCL17), regulated on activation normal T cell expressed and secreted (RANTES, CCL5), growth-regulated alpha (GRO-α, CXCL1), small inducible cytokine A1 (I-309, CCL1), interferon-gamma (IFN-γ), interferon-gamma inducible protein-10 (IP-10, CXCL10), and tumor necrosis factor (TNF-a).

Twenty de-identified serum specimens were obtained from volunteers at UHS-Wilson Memorial Hospital. Samples were stored at -20°C and underwent daily 1-hour thawing and subsequent freezing for each freeze-thaw cycle (FTC) over 49 consecutive days. Immunoregulator concentrations were assessed in participant samples at 2FTC (baseline), 25FTC, and 50FTC.


This project was funded by Binghamton University Harpur Edge and Binghamton University Research Awards.

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Creative Commons Attribution-NonCommercial 4.0 International License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Available for download on Friday, January 31, 2025