B cells Using Calcium Signaling for Specific and Rapid Detection of Escherichia coli O-157:H-7
food-borne pathogens; united-states; foodborne illness; biosensor;receptor; assay; activation; o157-h7; immunosensor; components
A rapid and sensitive detection technology is highly desirable for specific detection of E. coli O-157:H-7, one of the leading bacterial pathogens causing foodborne illness. In this study, we reported the rapid detection of E. coli O-157: H-7 by using calcium signaling of the B cell upon cellular membrane anchors anti-E. coli O-157: H-7 IgM. The binding of E. coli O-157:H-7 to the IgM on B cell surface activates the B cell receptor (BCR)-induced Ca2+ signaling pathway and results in the release of Ca2+ within seconds. The elevated intracellular Ca2+ triggers Fura-2, a fluorescent Ca2+ indicator, for reporting the presence of pathogens. The Fura-2 is transferred to B cells before detection. The study demonstrated that the developed B cell based biosensor was able to specifically detect E. coli O-157:H-7 at the low concentration within 10 min in pure culture samples. Finally, the B cell based biosensor was used for the detection of E. coli O-157:H-7 in ground beef samples. With its short detection time and high sensitivity at the low concentration of the target bacteria, this B cell biosensor shows promise in future application of the high throughput and rapid food detection, biosafety and environmental monitoring.
Wang, L., Wang, R., Kong, B. W., Jin, S., Ye, K., Fang, W., & Li, Y. (2015). B cells Using Calcium Signaling for Specific and Rapid Detection of Escherichia coli O157: H7. Scientific reports, 5. doi: doi:10.1038/srep10598