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In recent years “forever chemicals” have received much attention for the potential to cause harmful cancers, hormonal damage, and developmental issues. These chemicals can be found in a wide variety of products including clothing, which is particularly concerning as it allows them to come in direct contact with human skin. Perfluorooctanoic Acid (PFOA) is among this group and was the primary compound investigated due to its widespread use in clothing. A highly differentiated three dimensional skin model derived from Normal Human Epidermal Keratinocytes (NHEK) was exposed to this chemical at varying time intervals. Dermal irritancy induced by PFOA was evaluated through an MTT-assay, which indicates cellular metabolic activity through a colorimetric assay. It was found that an introduction with these chemicals to the skin constructs reduces metabolic activity. Future trials must be completed to affirm whether the effects of PFOA are consistent in these models across varying exposure lengths.



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Effects of Perfluorooctanoic Acid on Dermal Irritancy in Three Dimensional Skin Constructs