Author ORCID Identifier

Nicholas Buss: https://orcid.org/0000-0002-4801-153X

Advisor

Jessica Hua

Document Type

Data Set

Publication Date

5-2020

Keywords

Microcystin-LR, Lithobates clamitans, Trematode, Host-parasite interactions

Abstract

Wildlife diseases are emerging at unprecedented rates. While there are likely several factors at play, human-mediated environmental alterations may play a significant role. Of growing interest is the effect that microcystin-LR (MC-LR), a cyanotoxin, may have on disease outcomes. In this study, using an amphibian-trematode model we examined (1) the lethal effects of MC-LR on cercariae of trematodes; (2) the sublethal effects of MC-LR exposure on the ability for trematodes to infect green frog tadpoles; and (3) the sublethal effects of MC-LR on green frog tadpole susceptibility to trematodes. We found that environmentally-relevant concentrations of MC-LR at 50, 100, and 500 ug L-1 increased cercariae rate of mortality (LC50-14h = 134.24 ug L-1). However, sublethal exposure of trematodes to 2 and 10 ug L-1 MC-LR did not alter their infectivity. Conversely, sublethal exposure of tadpoles to 2 ug L-1 increased their susceptibility to trematodes by 147%. However, 10 ug L-1 of MC-LR did not affect tadpole susceptibility to trematodes, indicating a non-linear response to sublethal MC-LR exposure. Overall, our findings suggest that high concentrations of MC-LR (50 ug L-1) have the potential to limit trematode transmission to amphibian hosts through MC-LR-induced mortality. However, at lower concentrations (< 10 ug L-1) MCLR's effect on tadpole-cercariae disease outcome is likely driven by its effect on the tadpole host. Collectively, this work highlights the need to consider how toxicants influence both host and parasite at multiple concentrations to better understand the impacts of cyanotoxins on disease dynamics.

Comments

This data set accompanies the following published article:

Buss, N., M. Wersebe, and J. Hua. 2018. Direct and indirect effects of a common cyanobacterial toxin on amphibian-echinostome dynamics. Chemosphere 220:731–737. doi: 10.1016/j.chemosphere.2018.12.160.

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