Document Type

Article

Publication Date

5-2014

Keywords

Ethanol ; Infant ; Kappa ; Opioid ; Rats

Abstract

Approximately 10 to 15% of women consume alcohol (ethanol [EtOH]) during pregnancy in the United States. Even low amounts of EtOH consumption during pregnancy can elicit long-term consequences. Prenatal experience with as few as 3 drinks has been associated with increase problem drinking in adulthood. Such effects are corroborated in rodents; however, the underlying neural adaptations contributing to this effect are not clear. In the current set of experiments, we investigated whether changes in EtOH responding following prenatal EtOH exposure involved kappa opioid receptor activation and expression.Methods
Sprague–Dawley rats were prenatally exposed to low levels of alcohol (1.0 g/kg) during late gestation (gestational days 17 to 20 [GD17–20]) via intragastric intubation of pregnant dams. Following birth, EtOH intake, kappa- and mu-opioid-induced place conditioning, and kappa opioid receptor expression in mesolimbic brain regions were assessed in infant rats (postnatal days 14 to 15 [PD14–15]) that were offspring of dams given EtOH, vehicle, or untreated, during pregnancy.ResultsAnimals exposed to prenatal alcohol drank more alcohol later in life and exhibited significant changes in the kappa opioid system. While control subjects found kappa opioid activation aversive, animals exposed to EtOH prenatally exhibited either no aversion or appetitive responding. Further analysis revealed that synaptosomal kappa opioid receptor expression was significantly decreased in brain areas implicated in responding to EtOH.Conclusions
Overall, these data suggest that prenatal EtOH affects kappa opioid function and expression and that these changes may be involved in increased drinking later in life.

Comments

Nizhnikov, M., Pautassi, R., Carter, J., Landin, J., Varlinskaya, E., Bordner, K., . . . Spear, N. (2014). Brief Prenatal Ethanol Exposure Alters Behavioral Sensitivity to the Kappa Opioid Receptor Agonist ( U 62,066 E ) and Antagonist (Nor‐ BNI ) and Reduces Kappa Opioid Receptor Expression. Alcoholism: Clinical and Experimental Research, 38(6), 1630-1638.

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