Date of Award
Gold, Nanoparticle, Raman, Biosensor, Multiplexing, SERS, CEA, NSE, Cancer
Chemistry (BA, BS)
Dr. Chuan-Jian Zhong
Science and Mathematics
Biochemical markers -- Diagnostic use ; Raman spectroscopy ; Cancer -- Diagnosis
Cancer has a huge impact on people across the world. In 2020, there were about 19.3 million cancer cases and approximately 10 million deaths globally. Early detection and diagnosis of cancer results in a higher rate of survival of the disease. A key challenge is the need for a highly sensitive technique for early cancer detection. Surface-enhanced Raman spectroscopy and scattering (SERS) is a technique that can address the challenge by detecting biomarkers such as Carcinoembryonic antigen (CEA) and Neuron-specific enolase (NSE), which provide diagnostic information for assessing cancer detection. This work focuses on the use of gold nanoprobes conjugated with Raman reporters and capture/detection antibodies for the SERS detection of multiple cancer biomarkers. The main objective is to develop a nanoprobe system that can accurately detect the presence of biomarkers in multicomponent solutions. The results demonstrated that with an increase in the biomarker (CEA or NSE) antigen concentration, there is an increase in the SERS peak intensity. The results also revealed the multiplexed detection capability when the two biomarkers co-exist in the sample. These findings suggest that the further development of this multiplexing capability with magnetic core gold shell nanoparticles may be beneficial for even greater amplification of the SERS detection signal by enhancing the sensitivity and lowering the detection limit.
Hakimi, Sydney D., "Functionalized nanoprobes for multiplexed surface enhanced raman spectroscopic detection of cancer biomarkers" (2023). Undergraduate Honors Theses. 22.
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