Combinatorial Anti-Cancer Effect of Polypurine Reverse Hoogsteen Hairpins against KRAS and MYC Targeting in Prostate and Pancreatic Cancer Cell Lines

Authors

Simonas Valiuska
Kayla K. Elder, Binghamton University–SUNYFollow
Steven J. McKay, Binghamton University–SUNYFollow
Carlos J. Ciudad
Véronique Noé
Tracy A. Brooks, Binghamton University--SUNYFollow

Document Type

Article

Publication Date

10-16-2024

Keywords

KRAS, MYC, PPRH, G-quadruplex, prostate cancer, pancreatic cancer

Abstract

Introduction: KRAS and MYC are proto-oncogenes that are strictly regulated in healthy cells that have key roles in several processes such as cell growth, proliferation, differentiation, or apoptosis. These genes are tightly interconnected, and their dysregulation can lead to cancer progression. We previously individually targeted these oncogenes using Polypurine Reverse Hoogsteen (PPRH) hairpins, mostly targeting the complementary strand of G-quadruplex-forming sequences. We validated them in vitro in different cancer cell lines with deregulated KRAS and/or MYC. In this work we focused on our understanding of the cooperative dynamics between these oncogenes, by investigating the combined impact of PPRHs targeting KRAS and MYC in pancreatic and prostate cancer cells.

Results: The combinations had a modulatory impact on the expression of both oncogenes, with transcriptional and translational downregulation occurring five days post-treatment. Out of the four tested PPRHs, MYC-targeting PPRHs, especially HpMYC-G4-PR-C directed against the promoter, showed a greater cytotoxic and expression modulation effect. When both KRAS- and MYC-targeting PPRHs were applied in combination, a synergistic reduction in cell viability was observed.

Conclusion: The simultaneous targeting of KRAS and MYC demonstrates efficacy in gene modulation, thus in decreasing cell proliferation and viability.

Comments

https://doi.org/10.3390/genes15101332

Publisher Attribution

Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Recommended Citation

Valiuska, Simonas; Elder, Kayla K.; McKay, Steven J.; Ciudad, Carlos J.; Noé, Véronique; and Brooks, Tracy A., "Combinatorial Anti-Cancer Effect of Polypurine Reverse Hoogsteen Hairpins against KRAS and MYC Targeting in Prostate and Pancreatic Cancer Cell Lines" (2024). Pharmacy Faculty Scholarship. 49.
https://orb.binghamton.edu/pharmacy_fac/49

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.